Product Details
Neomycin sulfate acts as both an aminoglycoside antibiotic and a calcium channel protein inhibitor. It attaches to prokaryotic ribosomes to block protein synthesis, displaying activity against both gram-positive and gram-negative bacteria. Furthermore, it hinders PLC (Phospholipase C) via binding with inositol phospholipids, reduces phosphatidylcholine-PLD function, and triggers Ca²⁺ mobilization and PLA2 activation in human platelets. Neomycin sulfate also stops DNase I–mediated DNA breakdown. It is mainly applied to prevent and manage bacterial skin infections and shows no efficacy against fungal or viral pathogens.
Neomycin sulfate Chemical Properties
| Melting point | >187°C (dec.) |
| Alpha | D20 +54° (c = 2 in H2O) |
| Bulk density | 640kg/m3 |
| Refractive index | 56 ° (C=10, H2O) |
| Fp | 56℃ |
| Storage temp | 2-8°C |
| Solubility | H2O: 50 mg/mL As a stock solution. Stock solutions should be filter sterilized and stored at 2-8°C. Stable at 37°C for 5 days. |
| Form | powder |
| Color | white to slightly yellow |
| PH | 5.0-7.5 (50g/l, H2O, 20℃) |
| Biological source | Streptomyces sp |
| Water Solubility | Soluble in water |
| Merck | 146,454 |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| InChIKey | OIXVKQDWLFHVGR-VTSVPHRWSA-N |
| LogP | -3.655 (est) |
| CAS DataBase Reference | 1405-10-3(CAS DataBase Reference) |
| EPA Substance Registry System | Neomycin sulfate (1405-10-3) |
Safety Information
| Hazard Codes | Xn,Xi |
| Risk Statements | 42/43-22 |
| Safety Statements | 23-36/37-45-22-36-24/25 |
| WGK Germany | 3 |
| RTECS | QP4375000 |
| F | 8 |
| TSCA | Yes |
| HS Code | 29419000 |
| Toxicity | LD50 oral in mouse: > 8gm |
Application
Neomycin sulfate is an aminoglycoside antibiotic derived from S. fradiae, which suppresses protein synthesis by binding to the small subunit of prokaryotic ribosomes. It blocks voltage-gated Ca²⁺ channels and serves as a strong inhibitor of Ca²⁺ release from the sarcoplasmic reticulum of skeletal muscle. Research indicates that neomycin sulfate reduces inositol phospholipid metabolism, phospholipase C, and phosphatidylcholine–phospholipase D activity (IC50 = 65 μM). It exerts strong antibacterial effects against both Gram-positive and Gram-negative bacteria and is frequently employed to avoid microbial contamination in cell culture systems.
